Spectroanalysis in native gels ( SING ): rapid spectral analysis of pigmented thylakoid membrane complexes separated by CN – PAGE
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چکیده
منابع مشابه
Analysis of membrane protein complexes by blue native PAGE.
Blue native polyacryamide gel electrophoresis is a special case of native electrophoresis for high resolution separation of enzymatically active protein complexes from tissue homogenates and cell fractions. The method is powerful between 10 and 10,000 kDa. Also membrane protein complexes are separated well after solubilization of complexes with mild neutral detergents. The separation principle ...
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Gel-based analysis of thylakoid membrane protein complexes represents a valuable tool to monitor the dynamics of the photosynthetic machinery. Native-PAGE preserves the components and often also the conformation of the protein complexes, thus enabling the analysis of their subunit composition. Nevertheless, the literature and practical experimentation in the field sometimes raise confusion owin...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 2017
ISSN: 0960-7412,1365-313X
DOI: 10.1111/tpj.13703